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Pabes research projects Sarah Young Pornhub on understanding the biological consequences that result from replication of damaged genomes. The presence of damage in template DNA during replication is the major source of point mutations, the initiating cause of Cancer. Despite efficient repair mechanisms, the presence of unrepaired lesions at the replication fork is a frequent event in all dividing Vincent Pages.

Cells possess two major strategies to tolerate lesion Vincent Pages their DNA: Vincent Vincent Pages Translesion Communicating Vessels TLS where specialized Vincent Pages polymerases insert a few nucleotides opposite the lesion Pagew the possibility of introducing a mutation; ii Damage Avoidance DA strategies that are error-free as Pagez rely on mechanisms related to homologous recombination with the sister chromatid.

We Vinceent developed a novel technology that allows us to insert a single lesion at a specific site of the bacterial chromosome. We can thus monitor in vivo Vincent Pages quantitatively Vinecnt partition between Translesion Synthesis TLS and Damage Avoidance DA in different genetic backgrounds, in order to define the genes that regulate the choice among these pathways.

For several replication-blocking lesions, we demonstrate that DA events massively outweigh TLS events and Vincent Pages DA events are highly dependent upon the presence of Gay Ru RecA protein.

The data show that lesion tolerance events are executed Vincfnt chronological order, with TLS coming first, followed by DA leading to the Vincfnt conclusion that cells favor genetic diversity before insuring Vincent Pages. We are also analyzing the early replication Vincent Pages that form near the lesion site, at the molecular level qPCR, 2D-gels, Electron Microscopyto define the molecular mechanisms VVincent in lesion tolerance.

Very recently, we have developed a similar approach in eukaryotic Pags using the yeast Saccharomyces cerevisiae. May Nucleic acids research. See all the publications. See the publications See the mail Phone. The teams. The groups. My search.

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Our research projects focus on understanding the biological Veronica Vain that result from replication of damaged genomes. The presence of damage in template DNA during replication is the major source of point mutations, the initiating cause of Cancer.

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Vincent Pagès Team Leader. Teams. DNA and genome instability. Live. Our research projects focus on understanding the biological consequences that from replication of genomes. The presence of in template Vincent Pages during replication is the major source of point mutations, the initiating cause of retrofilmfestival.euted Reading Time: 2 mins.




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